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LS23L Final Exam Review 2025 Questions and Answers SDS, which stands for sodium dodecyl sulfate, is an anionic _______; it may break down _______ bonds. - --Answer --detergent; dipole-dipole You are conducting a gel electrophoresis to determine small differences in protein subunit molecular weights. Which of the following is a valid reason to choose a polyacrylamide gel over an agarose gel for your purposes? - --Answer -- Polyacrylamide has a higher resolution than agarose and will be able to distinguish smaller differences in sizes. What is the normal function of the protein product encoded by the I gene? - -Answer --Binds to the operator of the lac operon B-galactosidase activity, as seen by the cleavage of ONPG to o-nitrophenol, is clearly visible at the 70 minute mark, but not the 20 minute mark because: - -Answer --transcription and translation of b-galactosidase is time dependent, and enough of it needs to build up before it can cleave enough ONPG to produce visible amounts of o-nitrophenol (yellow). ....COPYRIGHT ©️ 2025 ALL RIGHTS RESERVED...TRUSTED & VERIFIED 1